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German to English translations [PRO] Science / Bioburden Testing
German term or phrase:Verdünnungsstufe
"Die Prüflösung wird vollständig von den Prüfmustern entfernt, in Zehnerschritten verdünnt und die Verdünnungsstufen wie üblich auf die Membranfilter aufgetragen und eine Keimzahlbestimmung auf Caso-Nährböden und Sabouraud-Agar (35°C/21°C) durchgeführt."
This is talking about determining a bioburden and bacterial count on a pharmaceutical product.
I can't work out the meaning of the "-stufe" part. Is it related to the "Zehnerschritte" or does it mean something like layers? TIA for helping me clear this up.
Explanation: it relates to the Pruefloesung which is removed from the Pruefmuster then diluted 10x further each time , each diluted part placed onto the membrane filter...
It seems as if the Muster contains something solid that has to be removed fist ; its the goodies in the solution they are after,presumably to get the bug content on the Agar membrane filters .
Wolf Brosius Local time: 20:01 Native speaker of: German, English PRO pts in pair: 607
Explanation: I would say you could translate it just as dilutions (as the Stufe part in English is contained with the meaning here). Clearly the dilutions are going to be of different concentrations- made more specific in German by the word Stufe
EST METHOD ESCHERICHIA COLI IN WATER BY THE MEMBRANE FILTER ...
Dateiformat: PDF/Adobe Acrobat - HTML-Version
... 2.4 Since a wide range of sample volumes or dilutions thereof can be ... water based on
the development of colonies on the surface of the membrane filter ... www.inh2o.org/Articles/Wastewater Articles/ 1_1_escherichia%20coli%20test%20method.pdf
A 10% solution is made of the original sample (1 part original + 9 parts diluting liquid) = A. Then 10% solution is made of this (1 part A + 9 parts diluting liquid) = B. Another 10 % solution is made (1 part B + 9 parts diluting liquid) and so on.
Then a sample of original solution, A, B, c etc. are applied to the membrane filter.
Each of these solutions are levels of dilution. Degree of dilution if you gave the amount (original, 10%, 1%, 0.1% etc).