преобразуются/по всей длине

17:25 Sep 23, 2019
Russian to English translations [PRO]
Science - Biology (-tech,-chem,micro-) / молекулы нуклеиновых кислот преобразуются в гены антитела
Russian term or phrase: преобразуются/по всей длине
Добрый день! переводим патент, но никак не можем понять, что тут имеется в виду и как это передать на англ.
"молекулы нуклеиновых кислот, кодирующие домены VH или VL преобразуются в гены антитела по всей длине путем вставки в экспрессионный вектор, уже кодирующей константные домены тяжелой цепи (СН) или легкой цепи (CL)

в частности, не понятно, что тут подразумевается под "преобразуются", и как правильно передать "по всей длине" на английском

Заранее благодарим!
Artem Anisimov
Russian Federation
Local time: 03:21


Summary of answers provided
3converted along the entire length
Frank Szmulowicz, Ph. D.


Discussion entries: 3





  

Answers


6 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
converted along the entire length


Explanation:
In another aspect of the present invention, nucleic acid molecules encoding the variable domain of a heavy (VH) and / or light (VL) chains of the first or second binding domain can be "transformed" along the entire length of the antibody genes. In one embodiment of the invention, nucleic acid molecules encoding the VH or VL domains are converted into antibody genes along the entire length by insertion into an expression vector that already encodes the constant domains of the heavy chain (CH) or light chain (CL), respectively, so that the VH segment is functionally connected to the CH segment (s) in the vector and / or the VL segment is operatively connected to the CL segment in the vector. In another embodiment of the invention, nucleic acid molecules encoding the VH and / or VL domains are converted into genes along the entire length of the antibody by connecting, for example, ligation, a nucleic acid molecule encoding the VH and / or VL domains to a nucleic acid molecule encoding CH and / or CL domains using standard molecular biological methods. Nucleic acid molecules that encode the heavy and / or light chain throughout their length can then be expressed in the cell into which they were inserted. Nucleic acid molecules can be used to express large amounts of recombinant antibodies to CD47 and PD-L1. Nucleic acid molecules can be used to produce human antibodies, humanized antibodies, chimeric antibodies, bispecific antibodies, single-chain antibodies, immunoadhesins, diabodies, mutated antibodies, and derivatives of antibodies, as described herein.

https://patents.google.com/patent/WO2019068302A1/en

--------------------------------------------------
Note added at 11 mins (2019-09-23 17:36:58 GMT)
--------------------------------------------------

This English text also uses "transformed" in quotation marks, corresponding to the use of «преобразовываться» in the Russian text.
https://patents.google.com/patent/WO2019068302A1/ru

Frank Szmulowicz, Ph. D.
United States
Local time: 19:21
Works in field
Native speaker of: Native in EnglishEnglish, Native in PolishPolish
PRO pts in category: 16
Login to enter a peer comment (or grade)



Login or register (free and only takes a few minutes) to participate in this question.

You will also have access to many other tools and opportunities designed for those who have language-related jobs (or are passionate about them). Participation is free and the site has a strict confidentiality policy.

KudoZ™ translation help

The KudoZ network provides a framework for translators and others to assist each other with translations or explanations of terms and short phrases.


See also:

Your current localization setting

English

Select a language

Term search
  • All of ProZ.com
  • Term search
  • Jobs
  • Forums
  • Multiple search