Siembra directa

English translation: direct seeding

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
Spanish term or phrase:siembra directa
English translation:direct seeding
Entered by: Neil Ashby

17:17 Apr 12, 2018
Spanish to English translations [PRO]
Medical - Medical: Pharmaceuticals
Spanish term or phrase: Siembra directa
Siembra directa

Primero se debe preparar el medio de cultivo a utilizar. Para ello fundirlo y enfriar a 40-45 ºC. Luego sembrar 1 ml por duplicado en dos placas de petri estériles. Plaquear un control negativo (sin muestra de agua).

Solo encontré no-till farming pero creo que no tiene que ver con esto....
sebaspedlp
Argentina
Local time: 16:43
direct seeding
Explanation:

by CM Ryu - ‎2005 - ‎Cited by 153 - ‎Related articles
287 above. Plastic Petri-dishes (100 × 15 mm) were pre- pared with MS solid media, ... For the direct seeding method, 1000 mL of 109 cfu/mL PGPR suspension were .... Effect of PGPR on growth of A. thaliana directly seeded or transplanted into soilless growth media under greenhouse condition seven weeks after seeding.



A comparative study of seeding techniques and ... - Wiley Online Library
onlinelibrary.wiley.com/doi/10.1002/term.302/pdf

Jul 26, 2010 - centre of the scaffold, prior to incubation in Petri dishes. (Fromstein et al. .... to the incubation period within the vacuum chambers, the scaffolds were transferred to untreated six-well non- tissue culture plates with 10 ml medium/well. The plates were placed on a ..... to direct seeding techniques. In contrast ...



Cor.4U® in xCELLigence® RTCA CardioECR assays - Ncardia
https://ncardia.com/files/.../Protocol_Cor.4U_xCELLigence_RT...

serum containing Cor.4U® Culture Medium for recovery and attachment to the culture surface. Pre-cultured cells in flasks are delivered with Axio-Supplement. Axio- ... Cor.4U®. (Ax-B-HC02-4M). 1. 4 million cells for direct seeding or pre- culture in T75 flask, enough for two E-. Plate CardioECR 48. Pre-cultured. Cor.4U®.


Does the volume of media affect the growth of cells in ... - ResearchGate
https://www.researchgate.net/.../Does_the_volume_of_media_af...

Get expert answers to your questions in Cell Culture, Animal Cell Culture and Culture Media and more on ResearchGate, the professional network for scientists. ... On the other hand, I change the media volume according to the seeding density of the cells for adherent cells, 2 - 5 ml of media for a T25 flask. Best,. Ozgun.
[PDF]CELL CULTURE BASICS Handbook
https://www.vanderbilt.edu/viibre/CellCultureBasicsEU.pdf

Cell culture vessels (e.g., flasks, Petri dishes, roller bottles, multi-well plates) ! Pipettes and ...... shake flask at a seeding density of 3 × 105 viable cells/mL in Sf-900 II SFM medium and it was maintained in a 28°C, non-humidified ..... Remove and discard the spent cell culture media from the culture vessel. 2. Wash cells using ...
Selected response from:

Neil Ashby
Spain
Local time: 21:43
Grading comment
thank you so much
4 KudoZ points were awarded for this answer



Summary of answers provided
3 +4direct seeding
Neil Ashby


  

Answers


1 hr   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +4
direct seeding


Explanation:

by CM Ryu - ‎2005 - ‎Cited by 153 - ‎Related articles
287 above. Plastic Petri-dishes (100 × 15 mm) were pre- pared with MS solid media, ... For the direct seeding method, 1000 mL of 109 cfu/mL PGPR suspension were .... Effect of PGPR on growth of A. thaliana directly seeded or transplanted into soilless growth media under greenhouse condition seven weeks after seeding.



A comparative study of seeding techniques and ... - Wiley Online Library
onlinelibrary.wiley.com/doi/10.1002/term.302/pdf

Jul 26, 2010 - centre of the scaffold, prior to incubation in Petri dishes. (Fromstein et al. .... to the incubation period within the vacuum chambers, the scaffolds were transferred to untreated six-well non- tissue culture plates with 10 ml medium/well. The plates were placed on a ..... to direct seeding techniques. In contrast ...



Cor.4U® in xCELLigence® RTCA CardioECR assays - Ncardia
https://ncardia.com/files/.../Protocol_Cor.4U_xCELLigence_RT...

serum containing Cor.4U® Culture Medium for recovery and attachment to the culture surface. Pre-cultured cells in flasks are delivered with Axio-Supplement. Axio- ... Cor.4U®. (Ax-B-HC02-4M). 1. 4 million cells for direct seeding or pre- culture in T75 flask, enough for two E-. Plate CardioECR 48. Pre-cultured. Cor.4U®.


Does the volume of media affect the growth of cells in ... - ResearchGate
https://www.researchgate.net/.../Does_the_volume_of_media_af...

Get expert answers to your questions in Cell Culture, Animal Cell Culture and Culture Media and more on ResearchGate, the professional network for scientists. ... On the other hand, I change the media volume according to the seeding density of the cells for adherent cells, 2 - 5 ml of media for a T25 flask. Best,. Ozgun.
[PDF]CELL CULTURE BASICS Handbook
https://www.vanderbilt.edu/viibre/CellCultureBasicsEU.pdf

Cell culture vessels (e.g., flasks, Petri dishes, roller bottles, multi-well plates) ! Pipettes and ...... shake flask at a seeding density of 3 × 105 viable cells/mL in Sf-900 II SFM medium and it was maintained in a 28°C, non-humidified ..... Remove and discard the spent cell culture media from the culture vessel. 2. Wash cells using ...

Neil Ashby
Spain
Local time: 21:43
Specializes in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 108
Grading comment
thank you so much

Peer comments on this answer (and responses from the answerer)
agree  Marie Wilson: Sounds right.
8 mins
  -> Thanks Marie

agree  Muriel Vasconcellos: I have often seen the phrase in English-original texts.
2 hrs
  -> Thanks Muriel

agree  neilmac
14 hrs
  -> Thanks Neil

agree  Chema Nieto Castañón
22 hrs
  -> Gracias Saltasebes
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