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METODO ENZIMATICO DE PUNTO FINAL

English translation: ENDPOINT ENZYMATIC METHOD

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GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
Spanish term or phrase:METODO ENZIMATICO DE PUNTO FINAL
English translation:ENDPOINT ENZYMATIC METHOD
Entered by: Patricia Luna
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22:34 Oct 21, 2002
Spanish to English translations [PRO]
Medical
Spanish term or phrase: METODO ENZIMATICO DE PUNTO FINAL
eSTOY TRADUCIENDO UN ANALISIS CLINICO DEL CASTELLANO AL INGLES Y EN EL HEMOGRAMA SALE UN ITEM UE SE LLAMA METODO ENZIMATICO DE PUNTO FINAL QUE SE USA PARA DETERMINAR EL CONTEO DE TRIGLICERIDOS
Patricia Luna
Local time: 13:48
Endpoint enzymatic assay (method)
Explanation:
un contexto:

Cobas Mira™ S Endpoint Enzymatic Assay for Plasma Formate*
B. Blomme[1] , P. Lheureux[2], E. Gerlo[1], and V. Maes[1]
[1]Department of Clinical Chemistry–Toxicology, Academic Hospital Free University Brussels, VUB, Laarbeeklaan 101, B-1090 Brussels, Belgium and
[2]Emergency Department, Erasme Hospital, ULB, Route de Lennik 808, B-1070 Brussels, Belgium

In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of sample is incubated for 5 min with buffer containing nicotinamide-adenine dinucleotide. Fifteen microliters of a suspension of formate dehydrogenase is then added. Absorbance at 340 nm is measured every 25 s. The NADH produced when formate is oxidized is stoichiometric to the amount of formate. The method is sensitive, reproducible, and specific and has a broad measurement range. The frozen reagents are stable for at least six months, so the described method can be applied to irregular and semi-urgent requests. A recent case is reported.



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Note added at 2002-10-21 22:47:40 (GMT)
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Method: Enzymatic Endpoint
Reaction Temperature: 37°C
Expected Values:
Desirable Range < 200 mg/dL
Borderline High Range 200 - 239 mg/dL
High Range > 240 mg/dL


--------------------------------------------------
Note added at 2002-10-21 22:48:00 (GMT)
--------------------------------------------------

http://www.thermodma.com/Products/cholesterol.htm
Selected response from:

Cinta Cano Barbudo
Spain
Local time: 18:48
Grading comment
MUCHAS GRACIAS!!
4 KudoZ points were awarded for this answer

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Summary of answers provided
4 +2Endpoint enzymatic assay (method)
Cinta Cano Barbudo


  

Answers


11 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +2
Endpoint enzymatic assay (method)


Explanation:
un contexto:

Cobas Mira™ S Endpoint Enzymatic Assay for Plasma Formate*
B. Blomme[1] , P. Lheureux[2], E. Gerlo[1], and V. Maes[1]
[1]Department of Clinical Chemistry–Toxicology, Academic Hospital Free University Brussels, VUB, Laarbeeklaan 101, B-1090 Brussels, Belgium and
[2]Emergency Department, Erasme Hospital, ULB, Route de Lennik 808, B-1070 Brussels, Belgium

In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of sample is incubated for 5 min with buffer containing nicotinamide-adenine dinucleotide. Fifteen microliters of a suspension of formate dehydrogenase is then added. Absorbance at 340 nm is measured every 25 s. The NADH produced when formate is oxidized is stoichiometric to the amount of formate. The method is sensitive, reproducible, and specific and has a broad measurement range. The frozen reagents are stable for at least six months, so the described method can be applied to irregular and semi-urgent requests. A recent case is reported.



--------------------------------------------------
Note added at 2002-10-21 22:47:40 (GMT)
--------------------------------------------------

Method: Enzymatic Endpoint
Reaction Temperature: 37°C
Expected Values:
Desirable Range < 200 mg/dL
Borderline High Range 200 - 239 mg/dL
High Range > 240 mg/dL


--------------------------------------------------
Note added at 2002-10-21 22:48:00 (GMT)
--------------------------------------------------

http://www.thermodma.com/Products/cholesterol.htm

Cinta Cano Barbudo
Spain
Local time: 18:48
Native speaker of: Native in ItalianItalian, Native in SpanishSpanish
PRO pts in pair: 51
Grading comment
MUCHAS GRACIAS!!

Peer comments on this answer (and responses from the answerer)
agree  Karina Pelech: assay .. perfecto ... :o)
6 mins
  -> gracias!!!!!

agree  rhandler
1 hr
  -> gracias Rhandler!
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