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Source text - English However, the heterotypy of methicillin resistance expression for coagulase-negative staphylococci, particularly S. epidermidis, is much greater than that seen for S. aureus. This results in a low minimal inhibitory concentration for methicillin or oxacillin, often below the accepted breakpoint for resistance for S. aureus.36 These isolates with low minimal inhibitory concentration values contain mecA37-39 and are fully resistant to all ß-lactam antibiotics when evaluated in animal models of infection.40,41 Because of the difficulty of detecting methicillin resistance in S. epidermidis, the oxacillin minimal inhibitory concentration breakpoints for defining resistance are lower than those determined for S. aureus.36,42 Identification of mecA by PCR amplification or PBP2A, the low affinity penicillin-binding protein encoded by mecA, by slide agglutination is the most sensitive and specific resistance-detection method.37,39,43
Translation - Spanish Sin embargo, la heterotipia en la expresión de resistencia a la meticilina por parte de los estafilococos coagulasa-negativos, especialmente S. epidermidis, es mucho mayor que la observada en S. aureus. Esto da lugar a que sean bajas las concentraciones inhibitorias mínimas de meticilina u oxacilina, con frecuencia inferiores al umbral de resistencia aceptado para S. aureus.36 Tales cepas con baja concentración inhibitoria mínima contienen mecA37-39 y resultan completamente resistentes a los ß-lactámicos en estudios con modelos animales de infección.40,41 Debido a la dificultad de detectar resistencia a la meticilina en S. epidermidis, el umbral de concentración inhibitoria mínima de oxacilina que define tal resistencia es inferior al de S. aureus.36,42 La identificación de mecA mediante amplificación de PCR o la PBP2A, proteína de unión a la penicilina de baja afinidad codificada por mecA, a través de aglutinación en portaobjetos, constituye el método más sensible y específico de detección de resistencia.37,39,43
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PhD - Universidad de Alcalá de Henares
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Years of experience: 35. Registered at ProZ.com: Jan 2007.
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