English to Russian Medical Medical (general)

The assay tubes were incubated for 60 min at room temperature after which time the tube contents were rapidly filtered under vacuum through GF/C filters presoaked for 60 min with PEI (0.3 %) with 2 x 2 ml washes of HEPES buffer [(50] mM, pH 7.4). The radioactivity retained on the filters was measured by scintillation counting. All assays were performed in triplicate in at least 2 separate experiments.

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и опять мне попалось предложение трудное для перевода ...