English to Polish Tech/Engineering Chemistry; Chem Sci/Eng analityka

Jakaś płytka z 96 zagłębieniami (ta liczba chyba nie jest istotna) do badania aktywności izozymów.

The assay is performed in a 96-well polypropylene microtiterplate (Costar 3794) that has been previously siliconized with Sigmacote (Sigma SL-2). The reaction mixture (50 ml) contains
10 ml of the relevant PKC isozyme together with 25 ml of the test compound and 15 ml of a mix solution that contains 200 mg/ml protamine sulfate, 10 mM Mg(NO3)2, 10 mM ATP (Boehringer 519987) and 3750 Bq of 33P-ATP (Hartmann Analytic SFC301, 110TBq/mmol) in 20 mM Tris-buffer pH 7.4 + 0.1% BSA.