English to Russian Law/Patents Biology (-tech,-chem,micro-) выращивание аденовирусов

The alternative ITR sequence was introduced into the left ITR by cloning into pAdapt and into the right ITR by cloning into pBr plasmids for Ad35 and Ad5, respectively (see e.g. Havenga M. et al, 2006, J. Gen.
Virol.87:2135-2143; Havenga M. et al, 2001, J. Virol.75:3335-3342).
To introduce the alternative ITR sequence into the left ITR, a fusion PCR was performed using a forward primer containing a ScaI site (GTGACTGGTGAGTACTC [SEQ ID NO:1]), a reverse primer containing an AvrII site (GACCACCTAGGCTGAC [SEQ ID NO:2]) and fusion forward and reverse primers harboring the alternative ITR sequence (alt ITR for 1:
TTAATTAATCGATCTATCTATATAATATACCTTATAG [SEQ ID NO:3] , alt ITR for 2:GATCTATCTATATAATATACCTTATAGATGGAATGG [SEQ ID NO:4], alt ITR rev:
ATTATATAGATAGATCGATTAATTAATTCGAACCC [SEQ ID NO:5]).
Two partly overlapping ITR forward primers were used in the PCR for one of the fusion PCR fragments to increase the PCR efficiency on an extremely AT rich region in the template.
The fusion PCR product was first subcloned into the pTopo vector to facilitate sucloning and then inserted into pAdapt35 plasmid via the AvrII and ScaI sites with the indicated transgenes.
To introduce the alternative ITR sequence into the right ITR, a fusion PCR was performed using a forward primer containing an NdeI site and a reverse primer containing an NruI site and fusion forward and reverse primers harboring the alternative ITR sequence using the same fusion PCR strategy as described for the left ITR.


Проблема в том, что сперва я подумал об использовании в данном методе лишь fusion-полимеразы, но далее по тексту идет указание на использование fusion forward and reverse primers.
Правильным ли будет перевод - ПЦР с использование fusion-полимеразы (или гибридизованной полимеразы - вот на сайте производителя она хорошо описана - http://www.genomics.agilent.com/article.jsp?crumbAction=push...
Как при этом правильно перевести fusion forward and reverse primers?

Спасибо!