English to French Science Patents Thrombospondin fragments

The present results provide validation for other fragment-based approaches, including (but not limited to) non-

competitive ELISA and ELISA-like assays, and competition assays.

Figure 4 shows the results of an independent gel analysis of the samples. The samples were denatured then

run on a 12% gel, transblotted, and then stained with the same TSP Ab-4 that we used before. Unlike the blot shown

in Figure 3, the denaturation step here included urea, and detection used an enzymatic colorometric method that is

based on horseradish peroxidase conjugates and the BioRad Opti-4CN substrate kit