English to Spanish: INFORME DE ENSAYO EVALUACIÓN DE LA ACTIVIDAD VIRUCIDA DE LA FÓRMULA General field: Medical Detailed field: Medical: Instruments
Source text - English c) Elimination of the cytotoxicity / Elimination de la cytotoxicité
For elimination of cytotoxicity of the disinfectant solutions, eight parts by volume of solutions of disinfectant mixed with two parts by volume of water for injection, were filtrated on Microspin S-400 HR column (GE Healthcare). Then, filtrates were diluted from 10-1 to 10-8 with ice-cold culture medium. 0.1 ml. of each dilution was transferred, into 4 wells of a 96 wells micro titre plate with an established cell monolayer. Microtitre plates were incubated at 37ºC in a 5% COw -atmosphere. 1 h after 0.1 ml of culture medium was added to each well. The cytotoxic effect was read after incubation during 5 and 7 days in a 5% Co2-atmosphere.
Les solutions d'essai de la formule additionnées de 1/5 éme d'eau pour préparaton injectable, sont filtrées sur colonne Microspin S-400 (GE Healthcare). Les filtrats sont ensuite dilués de 10-1 à 10-8 dans du milieu de cuture à 2% de SVF glacé. 0,1 ml de chaque dilution est distribuè dans4 puits d'une plaque 96 puits de microtritration contenan un tapis monocellulaire pré-établie. Après une incubation d'une heure à 37ºC en preésence de 5% de CO2, 0,1 ml de milieu de culture à 2% de SVF est ajouté aux puits. L'effet subcytotoxique est apprécié après une incubation de 5 ou 7 jours à 37ºC en prèsence de 5% de CO2.
Translation - Spanish c) Eliminación de la citotoxicidad de la fórmula
Para la eliminación de citotoxicidad de las soluciones desinfectantes, fueron filtrados 0,1 ml de ocho partes en volumen de desinfectante mezclada con una parte en volumen de la substancia interferente y una parte en volumen de agua para inyección, a través de una columna de Microspin S-400 HR (GE Healthcare). Los filtrados fueron entonces diluidos de 10-1 a 10-8 con medio DMEM + 2% de SFB helado. 0,1 ml de cada dilución fueron transferidos en 4 pocillos de una placa de 96 pocillos de microtitulación, conteniendo un tapis monocelular pre-establecido de células Hep2. Las placas de microtitulación fueron incubadas a 37ºC en una atmósfera de CO2 al 5%. Después de una hora de incubación, se adicionó a cada pocillo 0,1 ml de medio de cultivo. La lectura del efecto subcitotóxico se realizó después de 5 y 7 días de incubación a 37ºC en una atmósfera de CO2 al 5%.
Other - Anglo American School
Years of translation experience: 13. Registered at ProZ.com: Apr 2015. Became a member: Jun 2017.
I am a Peruvian, agriculture professional, with more than 12 years of experience in translation, editing, proofreading and interpreting.
I speak English since elementary school (also bilingüal High School) and I have worked as Head of Quality Assurance and Social Welfare in a seedling nursery, Sanitary Inspector and Producers Trainer in the Ministry of Agriculture, and as Translator and Interpreter in an Importer of Medical Devices and Products.
My fields of expertise have broadened to hospital healthcare, Medical Devices, QA, Social Projects, and Tourism fields, keeping satisfied customers for many years until now.
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