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Climate scorecard for the Vancouver 2010 Olympics (The David Suzuki Foundation)
Environment & Ecology
No comment.
Translation Volume: 0 days Languages: English to French
USDA-APHIS Decision on Monsanto Petition 04-086-01p Seeking a Determination of N
Genetics
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Translation Volume: 0 days Languages: English to French
Microsoft letter to Yahoo! Board of directors
Business/Commerce (general)
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Translation Volume: 0 days Languages: English to French
City of Edmonton bid for EXPO 2017
Advertising / Public Relations
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Blue Board entries made by this user
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English to French: excerpt from: USDA-APHIS Decision on Monsanto Petition 04-086-01p Seeking a Determination of Nonregulated Status for Glyphosate-Tolerant Cotton Line MON 88913 General field: Science Detailed field: Genetics
Source text - English The complete source text can be found at: http://www.aphis.usda.gov/brs/aphisdocs/04_08601p_pea.pdf
As in line 1445, MON 88913 was developed by using recombinant DNA techniques to introduce a gene for EPSPS (5-enolpyruvylshikimate-3-phosphate synthase), isolated from Agrobacterium tumefaciens strain CP4 that encodes an enzyme that confers tolerance to glyphosate, the active ingredient of Roundup® agricultural herbicides. As determined by Southern blot analysis, MON 88913 contains a single intact DNA insert from the binary plasmid PV-GHGT35 at a single integration locus within the cotton genome. While line 1445 contains one intact cp4 epsps gene expression cassette, the DNA insert in MON 88913 contains two intact cp4 epsps gene expression cassettes containing identical cp4 epsps coding sequences. Polymerase chain reaction was performed to confirm the 5' and 3' insert-to-genomic DNA junctions and the organization of elements within the insert in MON 88913. The DNA insert and the glyphosate tolerant trait are stable across multiple sexual generations. Phenotypic segregation data confirmed that the single insert locus and glyphosate tolerant trait behave as a single dominant locus with the expected Mendelian segregation pattern across multiple generations.
The CP4 EPSPS protein produced in MON 88913 is targeted to the chloroplasts via an N-terminal fusion with the chloroplast transit peptide, CTP2, to form a CTP2-CP4 ESPSP precursor protein. The precursor protein produced in the cytoplasm is processed to remove the transit peptide upon translocation into the plant chloroplast, resulting in the mature CP4 EPSPS protein. The transgenic cotton line that is the subject of the petition was developed by a widely used technique called Agro-infection which essentially involves using a plant pathogenic strain of Agrobacterium tumefaciens and its disarmed plasmid vector.
Translation - French Comme la lignée 1445, MON 88913 a été développé à l’aide de techniques de recombinaison de l’ADN afin d’introduire un gène exprimant l’EPSPS (5-enolpyruvylshikimate-3-phosphate synthase). Celui-ci a été isolé à partir de la souche CP4 de l’Agrobacterium tumefaciens qui code un enzyme conférant la tolérance au glyphosate, l’ingrédient actif des herbicides agricoles Roundup®. Comme déterminé suite à l’analyse du transfert de Southern, MON 88913 contient un seul insert d’ADN du plasmide binaire PVGHGT35 à un seul locus d’intégration dans le génome du coton. Tandis que la lignée 1445 contient une cassette d’expression cp4 epsps intacte, l’insert d’ADN dans le MON 88913 contient deux cassettes d’expression cp4 epsps intactes avec des séquences codantes cp4 epsps identiques. La réaction en chaîne de la polymérase a été menée afin de confirmer les jonctions aux extrémités 5’ et 3’ de l’ADN inséré et l’organisation des éléments dans l’insert de MON 88913. L’insert d’ADN et le caractère de tolérance au glyphosate sont stables pendant plusieurs générations sexuées. Les données de ségrégation phénotypique confirment que le locus d’insert unique et le caractère de tolérance au glyphosate agissent en tant que locus dominant unique avec le profil de ségrégation mendélienne attendu pendant plusieurs générations.
La protéine CP4 EPSPS créée dans MON 88913 cible les chloroplastes à l’aide d’une fusion N-terminal au peptide de transit du chloroplaste, CTP2, afin de former une protéine précurseur CTP2-CP4 ESPSP. La protéine précurseur créée dans le cytoplasme est transformée lors de sa translocation au chloroplaste et le peptide de transport est éliminé pour créer la protéine CP4 EPSPS mature. La lignée de coton transgénique qui fait l'objet de la pétition a été développée à l’aide d’une technique largement employée, nommée Agro-infection; celle-ci consiste à se servir d’une souche d’Agrobacterium tumefaciens, pathogène des plantes, et de son vecteur du plasmide désarmé.
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Translation education
Bachelor's degree - Concordia University
Experience
Years of experience: 18. Registered at ProZ.com: Sep 2007. Became a member: Mar 2008.
I am a Canadian French translator with a B.Sc. in Biology and a B.A. Specialized in Translations, both from Concordia University, in Montreal.
I have 16 years of translating experience, including 2 years as an in-house translator and editor (translation and vendor quality control).
I am a technical translator specialized in the Financial, Pharmaceutical, Engineering, Automotive and Public service fields. I offer translation, review and language quality assessment (LQA) services for French (Canada).
Translation & editing :
Pharmaceutical and medical devices : >5 million words
Finance : >3.5 million words
Engineering (bridges, roads, trains, mining) : >750,000 words
Automotive : >500,000 words
Public service, government : >350,000 words
LQA of MTPE in MQM / DQF-MQM format : >2 million words
Member since Mar '08
Expertise 
positive
