Glossary entry

English term or phrase:

nick translation labelling

Spanish translation:

marcaje por traslado/traslación/desplazamiento de mella

Added to glossary by Paula Mariani
Oct 26, 2004 12:10
19 yrs ago
10 viewers *
English term

nick translation labelling

English to Spanish Other Genetics
nick translation labelling
non coding sequence


GENETIC TERMS

Proposed translations

4 hrs
Selected

marcaje por traslado/traslación/desplazamiento de mella

nick translation A procedure for labelling DNA. A DNA fragment is treated with DNase to produce single-stranded nicks. The nick is moved along the DNA molecule in the presence of labelled deoxyribonucleoside triphosphates by the concerted action of the 5´-> 3´ exonuclease and 5´-> 3´ polymerase activities of E. coli DNA polymerase I.
nick Verb: To break a phospho-diester bond in the backbone of one of the strands of a duplex DNA molecule. cf cleave; cut.
labelling The process of replacing a stable atom in a compound with a radioactive isotope of the same element to enable it to be detected by autoradiography or other techniques. Increasingly, radioactive labelling is being replaced by fluorescent labelling. The method is used to trace the path of the labelled compound through a biological or chemical system.
http://www2.koko.gov.my/CocoaBioTech/Glossary.html

Genética molecular:
1.Electroforesis normal, Electroforesis de campo pulsante, Secuenciación de DNA en aparatos Li COR (doble cadena y cadena sencilla), Marcajes radiactivos y no radiactivos (Random-priming, Nick translation, Ready-Prime, etc...),
http://www.terra.es/personal/laurel/CV/Curred.htm

Técnicas de marcaje de ácidos nucleicos:
 Marcajes radiactivos.
 Autorradiografía.
 Marcajes fluorescentes.
 Marcajes colorimétricos.
 Método de marcaje de sondas genéticas.
http://www.xunta.es/conselle/as/spe/dxfc/sxfo/espec/SAXX01.H...

Tema 6.- Técnicas y estrategias de clonación.
- Las sondas en Ingeniería Genética. Sondas de ADN, algunos métodos de marcado: desplazamiento de mella (nick translation),
http://www.umh.es/asignaturas/fichasignatura.asp?asi=5790&AR...

nick translation: traslación de mellas
labelling: marcado
http://www.leeds.ac.uk/ifcc/PD/dict/dictn.html
http://www.leeds.ac.uk/ifcc/PD/dict/dictl.html

Marcaje por traslado de la mella (Univ. Udine)
http://www2.uah.es/bioquimica/f-bcpmh/contenido.htm

http://www2.udec.cl/~digentox/informacion/aingles.html



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Note added at 4 hrs 31 mins (2004-10-26 16:41:30 GMT)
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en lugar de marcaje puede ser marcado pero encontre como mas usado \"marcaje\"
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4 KudoZ points awarded for this answer. Comment: "Gracias!"
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