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Glossary entry

French term or phrase:

polynucléotide complémentaire inversé

English translation:

inverse complementary polynucleotide

Added to glossary by Louisa Tchaicha
Oct 9, 2010 20:01
13 yrs ago
French term

polynucléotide complémentaire inversé

French to English Science Biology (-tech,-chem,micro-) polynucléotides
Hi,

"Par "polynucléotide complémentaire inversé" ...on entend un nouveau polynucléotide,..."

isit "inverse complementary nucleotide" in English?

Thank you in advance :)
Proposed translations (English)
4 +2 inverse complementary polynucleotide
2 +1 antiparallel polynucleotide sequence
3 reverse complement (of a) polynucleotide
References
Finding only: http://webcache.googleu...
Change log

Oct 10, 2010 07:40: writeaway changed "Field" from "Law/Patents" to "Science"

Discussion

Lionel_M (X) Oct 10, 2010:
Par « polynucléotide complémentaire inversé » d'un polynucléotide donné, on entend un nouveau polynucléotide, existant ou théorique, où chaque nucléotide du polynucléotide donné est remplacé par un nucléotide complémentaire pouvant s'apparier avec le premier, comme par exemple une adénine remplaçant une thymine, ou une thymine remplaçant une adénine, ou une cytosine remplaçant une guanine, ou une guanine remplaçant une cytosine dans le cas d'un polynucléotide d'acide désoxyribonucléique. Par « hybridation » on entend l'association par des liaisons non covalentes de deux polynucléotides simple brin complémentaires. Cette l'hybridation peut être parfaite, c'est à dire que les séquences sont totalement complémentaires, ou imparfaite, c'est à dire que les séquences ne sont pas totalement complémentaires mais suffisamment complémentaires pour s'hybrider entre elles et former une structure double-brin.
Lionel_M (X) Oct 10, 2010:
@Liz Check at 14.30 and past it into Google :)
Sue also provide this link on another question of Loulou
liz askew Oct 10, 2010:
Thank you Lionel. Kindly provide the exact whereabouts of the phrase "polynucléotide complémentaire inversé" because the site you have quoted does not contain the highlighted phrase.
Lionel_M (X) Oct 10, 2010:
Link http://www.sumobrain.com/patents/wipo/Method-labelling-produ...
Lionel_M (X) Oct 10, 2010:
But... how many "Reverse complement polynucleotide" can you find?
2 GHITS !
Nevertheless it's widely used in PCR terminolgy !
liz askew Oct 10, 2010:
@Lionel, kindly provide the French site so I can access it and actually see "polynucléotide complémentaire inversé" as quoted in the actual query. Thank you!. Then I can see it for my own eyes, as I am damned if I can find it in google searching myself.
liz askew Oct 10, 2010:
We are at cross purposes, I am talking about the use of "inverse" in English. Why no native English sites with "inverse complementary polynucleotide" then? and why can I myself not find any French site with "polynucléotide complémentaire inverse/inversé?
Lionel_M (X) Oct 10, 2010:
@Liz It's not a translation since the brevet if French Liz !

Inventors:JACOB, Alexandre (440 avenue Maurice dauvergne, LE MEE SUR SEINE, , F-77350, FR)
KEUMEUGNI KWEMO, Carlosse (19 rue des Bourguignons, BOIS COLOMBES, , F-92270, FR)
LORIC, Sylvain (7 rue des Vallées, BRUNOY, , F-91800, FR)
MOUTEREAUX, Stéphane (1 rue Jean Magloire Piquet, MAROLLES EN BRIE, , F-94440, FR)
DELACOTTE, Nicolas (13 allée du Parc Bièvre, L'Hay -les- Roses, , F-94520, FR)
Application Number:FR2009/000422
Lionel_M (X) Oct 10, 2010:
@Sue If you want to redesign molecular biology OK !
liz askew Oct 10, 2010:
Well, it could be "strange" because it is a poor translation.
SJLD Oct 10, 2010:
No it doesn't. It's just a description of the sequence.
Lionel_M (X) Oct 10, 2010:
@Sue If you use "reverse complement" this technically means that the oligo is obtaines by reverse transcription. Ex: cDNA
Lionel_M (X) Oct 10, 2010:
@Liz If you read the brevet found by Use:
"Par « polynucléotide complémentaire inversé » d'un polynucléotide donné, on entend un nouveau polynucléotide, existant ou théorique..."

you'll see that the expression is between "guillemets"; nothing strange IMHO that you dnt find it in thousand sites :)
liz askew Oct 10, 2010:
Sorry, I have to ask too "why can I not find "inverse complementary polynucleotide" as a phrase either? or is this some recent discovery??
liz askew Oct 10, 2010:
Hi,I have to ask ...is it "inverse" or "inversé" [with an acute accent] and why can I not find this phrase on a French site?
SJLD Oct 10, 2010:
Where is the reference to reverse transcription in my answer?
Lionel_M (X) Oct 10, 2010:
Not a problem of stupidity, but the fact that you focussed your attention to polynuceotides obtained from REVERSE transcription and not by oligonucleotide synthesis.
SJLD Oct 10, 2010:
Well I'm just too stupid to understand I guess. Funny how the people who write textbooks are stupid too...
Lionel_M (X) Oct 10, 2010:
Sue, the problem is not FR and may be you're not aware of vocabulary in French molecular laboratories, but the difference between "inverse" and "reverse"; in molecular biology it's not the same thing ! I think Zareh and Joanne are very clear about too.
SJLD Oct 10, 2010:
There is only one ghit for that in the context of genetics and in French - the Belgian text you cite.
EPATRAS - Display Text
15 SEQ ID N°24 est une amorce réverse basée sur le complément réverse de SEQ ID N°22. SEQ ID N°25 est une amorce réverse basée sur le complément réverse de ...
epatras.economie.fgov.be/affTexte.jsp?id=/ExtraData/...

"Complément réverse" is quite simply wrong.
Read the links in my answer : "complémentaire inversé" = "reverse complement".
Lionel_M (X) Oct 10, 2010:
15 SEQ ID N°24 est une amorce réverse basée sur le complément réverse de SEQ ID N°22.
SJLD Oct 10, 2010:
What's "reverse complement" in French then?
Lionel_M (X) Oct 10, 2010:
@Sue Le problème ici est que l'on ne parle pas de PCR, mais de marquage d'oligo. Il n'est pas question de "reverse" car on n'utilise pas la "reverse transcritase". Le qualificatif "inverse" s'applique à la séquence de l'oligo; non au fait qu'il soit obtenu par reverse transcription/transcription inverse. C'est un problème de vocabulaire de biomol. Voir explication de Zareh
SJLD Oct 10, 2010:
@Lionel How do you translate "reverse complement" into French then?

And "transcription inverse" = reverse transcription, n'est-ce pas?
Lionel_M (X) Oct 10, 2010:
...suite On lit :

"La présente invention a précisément pour but de proposer une solution de marquage répondant à ce besoin et résolvant les problèmes de l'art antérieur. La présente invention se rapporte en particulier à un procédé de marquage d'un produit, ledit procédé comprenant une étape d'addition sur ou dans ledit produit d'une pluralité de polynucléotides simples brins, ladite pluralité de polynucléotides comprend :

- au moins un polynucléotide cible constitué d'un polynucléotide simple brin de longueur et de séquence déterminée, et

- des polynucléotides leurres qui sont de longueurs déterminées identiques ou différentes et de séquences déterminées identiques ou différentes, lesdits polynucléotides leurres étant de longueur(s) identique(s) ou différente(s) et de séquences différentes de la séquence dudit, au moins un, polynucléotide cible

dans lequel chacun des polynucléotides cible(s) et leurres ne s'hybride avec aucun des autres polynucléotides de ladite pluralité de polynucléotides. "



Il ne s'agit donc pas de "produit" obtenu par "reverse transcriptase" qui fournit une séquence "reverse complément". Mais d'une "inverse sequence", antiparallel to original seq.
Lionel_M (X) Oct 10, 2010:
Grace au lien reporté par SJLD: Par « polynucléotide complémentaire inversé » d'un polynucléotide donné, on entend un nouveau polynucléotide, existant ou théorique, où chaque nucléotide du polynucléotide donné est remplacé par un nucléotide complémentaire pouvant s'apparier avec le premier, comme par exemple une adénine remplaçant une thymine, ou une thymine remplaçant une adénine, ou une cytosine remplaçant une guanine, ou une guanine remplaçant une cytosine dans le cas d'un polynucléotide d'acide désoxyribonucléique. Par « hybridation » on entend l'association par des liaisons non covalentes de deux polynucléotides simple brin complémentaires. Cette l'hybridation peut être parfaite, c'est à dire que les séquences sont totalement complémentaires, ou imparfaite, c'est à dire que les séquences ne sont pas totalement complémentaires mais suffisamment complémentaires pour s'hybrider entre elles et former une structure double-brin.

.......
Lionel_M (X) Oct 10, 2010:
@Loulou Are you talking about PCR technique ? And do you refer to "cDNA" ?

Proposed translations

+2
30 mins
Selected

inverse complementary polynucleotide

Appropriate terminology for a literal patent translation...
Don't forget the poly part!

But if you have more flexibility in your sentence structure, you could use wording similar to this:

In a construct that comprises two copies of a desired polynucleotide, therefore, where one of the polynucleotides may or may not be oriented in the inverse complementary direction to the other, and where the polynucleotides are operably linked to promoters to induce convergent transcription...

The desired polynucleotides may be perfect or imperfect repeats of one another, or perfect or imperfect inverse complementary repeats of one another.
Reference:

http://www.wipo.int/pctdb/en/wo.jsp?KEY=07%2F035752&IA=US2006036515&DISPLAY=DESC

http://www.freepatentsonline.com/y2009/0220670.html

Peer comment(s):
agree Lionel_M (X) : aussi
1 hr
thanks
agree Zareh Darakjian Ph.D.
8 hrs
thanks
Something went wrong...
4 KudoZ points awarded for this answer. Comment: "Many thanks to everyone for all their help!"
+1
18 mins

antiparallel polynucleotide sequence

One strand is in the 5'->3' direction, the other in the 3'>5' direction. That's the "inverse" I would think. The complimentarity is in reference to GC, AT, pairing in the DNA.
Peer comment(s):
agree Lionel_M (X) : OH YES !!!!!!!!!!!!!!!!!!
1 hr
Thank you, Lionel!
Something went wrong...
27 mins

reverse complement (of a) polynucleotide

In genetics, "inversé" in FR is usally "reverse" in English.

(WO/2007/024808) POLYNUCLEOTIDES TEMOINS INDICATEURS - [ Traduire cette page ]
In one embodiment the IPC method designs indicator polynucleotide controls that include reverse polynucleotides, reverse complement polynucleotides, ...
www.wipo.int/pctdb/fr/wo.jsp?WO...IA... - En cache(WO/2004/040000) G PROTEIN COUPLED RECEPTORS AND USES THEREOF - [ Traduire cette page ]
A substantially pure polynucleotide having at least 90% sequence identity to the reverse complement of polynucleotide listed in Table 2. ...
www.wipo.int/pctdb/en/wo.jsp?KEY=04/040000... - En cachePlus de résultats de www.wipo.intIndicator polynucleotide controls - [ Traduire cette page ]
de J Bingham - 2006
1 Mar 2007 ... In one embodiment the IPC method designs indicator polynucleotide controls that include reverse polynucleotides, reverse complement ...
www.freepatentsonline.com/y2007/0048764.html - En cacheMethods and Reagents for Polynucleotide Assembly - Patent ... - [ Traduire cette page ]
de AY Borovkov - 2007 - Autres articles
10 Dec 2009 ... Determining the boundaries of overlap segments in the target polynucleotide sequence or part thereof and its reverse complement; and; 6. ...
www.freepatentsonline.com/y2009/0305233.html - En cachePlus de résultats de www.freepatentsonline.comPatent applications filed by Syngenta Participations AG - [ Traduire cette page ]
f) the reverse complement of any sequence of a), b), c) or d); g) a polynucleotide encoding any polypeptide selected from the group consisting of SEQ ID NOs ...
www.patentlens.net/.../1725.html - En cache - Pages similairesISOLATED SNARE YKT6 GENOMIC POLYNUCLEOTIDE FRAGMENTS FROM ... - [ Traduire cette page ]
[0018](g) a polynucleotide that is a reverse complement to the polynucleotides specified in (a)-(f) and [0019](h) containing at least 10 transcription ...
www.faqs.org/patents/app/20100081709

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Note added at 10 hrs (2010-10-10 07:00:03 GMT)
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http://books.google.fr/books?id=unhSc7Jj23QC&pg=PA238&lpg=PA...

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Note added at 11 hrs (2010-10-10 07:13:54 GMT)
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http://www.lifl.fr/~noe/enseignements/iup-genpro/TPs/tp_comp...

Réaliser un nouveau dotplot (même parametres) en comparant la séquence originale contre son complémentaire inversé (utilisez le programme revseq disponible sur Mobyle).

Programme revseq:
http://emboss.bioinformatics.nl/cgi-bin/emboss/revseq

revseq
Reverse and complement a nucleotide sequence (read the manual)


--------------------------------------------------
Note added at 12 hrs (2010-10-10 08:20:56 GMT)
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WO/2007/095387) COMPOSITIONS ET PROCÉDÉS PERMETTANT L'INHIBITION ...
... région complémentaire inverse (RC) à la molécule d'intérêt cible (par exemple, ... The RNAi inhibitors of the invention have a reverse complement (RC) ...
www.wipo.int/pctdb/fr/wo.jsp?WO=2007095387 -

... which have the sequences CGGCC or the reverse complement GGCCG; ... 1 en amont possédant la séquence CGGCC ou la séquence complémentaire inverse GGCCG. ...
www.surechem.org/index.php?Action...
Peer comment(s):
disagree Lionel_M (X) : reverse NON ! Pas de place pour expliquer la différence entre "inverse" et "reverse" sorry
1 hr
http://imgt.cines.fr/textes/IMGTmedical/sequence_analysis.ht... + http://tinyurl.com/3yxo55b
agree Joan Berglund : I really do think reverse is the better term here, although inverse is used. Reverse does not need to have anything to do with reverse transcription, it is just the reversed or inverted sequence
18 hrs
yes, inverse and reverse are frequently interchanged, but I think reverse is better too ;-)
neutral liz askew : Profound apologies, but I have had to sit on the fence here as further linguistic research. //What else can I do if I am a medical linguist, and not a scientist or medic??
18 hrs
thanks Liz :-)/perhaps best not to base medical/scientific translations solely on linguistic criteria :-)
Something went wrong...

Reference comments

19 hrs
Reference:

Finding only:

http://webcache.googleusercontent.com/search?q=cache:1KlhBn0...

In another embodiment the IPC method comprises providing an indicator polynucleotide control that includes reverse polynucleotides, reverse complement polynucleotides, or complement polynucleotides for Exon A Indicator, Exon B


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Note added at 19 hrs (2010-10-10 15:26:03 GMT)
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seriously
I am unable to find one single French hit for the phrase "polynucléotide complémentaire inverse/inversé"

How odd

--------------------------------------------------
Note added at 19 hrs (2010-10-10 15:31:07 GMT)
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and unless I am going blind, I am unable to find a single English site with "inverse complementary polynucleotide".

--------------------------------------------------
Note added at 19 hrs (2010-10-10 15:34:37 GMT)
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#
ISOLATED SNARE YKT6 GENOMIC POLYNUCLEOTIDE FRAGMENTS FROM ...
[0018](g) a polynucleotide that is a reverse complement to the polynucleotides specified in (a)-(f) and [0019](h) containing at least 10 transcription ...
www.faqs.org/patents/app/20100081709 - Cached
#
Gene Cloning and DNA Analysis: An Introduction - Google Books Result
Terry A. Brown - 2010 - Science - 336 pages
3′′ terminus One of the two ends of a polynucleotide: that which carries the hydroxyl ... Antisense RNA An RNA molecule that is the reverse complement of a ...
books.google.co.uk/books?isbn=1405181737...

--------------------------------------------------
Note added at 19 hrs (2010-10-10 15:57:34 GMT)
--------------------------------------------------

and findings for

reverse complementary repeats

#
Cytoplasmic ribosomal protein genes of the fission yeast ...
16 Sep 2010 ... The tandem repeat AGGGTAGGGT of Homol E was found as AGGGTAGGGC or as AGGTTAGGGT, whereas the reverse complementary repeat ACCCTACCCT was ...
nar.oxfordjournals.org/content/26/14/3319.full
# [PDF]
Compression of Strings with Approximate Repeats
File Format: PDF/Adobe Acrobat - Quick View
by L Allison - 1998 - Cited by 20 - Related articles
imate reverse complementary repeats when analyzing DNA strings. Tests include the recovery of ..... Reverse complementary repeats were left out of the dis- ...
citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.14...
#
Forensic DNA typing: biology & technology behind STR markers - Google Books Result
John M. Butler, John Marshall Butler - 2001 - Law - 322 pages
... equivalent to motif B when (1) motif A is inversely complementary to motif B, ... For reasons that will be discussed below, tetranucleotide repeats have ...
books.google.co.uk/books?isbn=012147951X...
#
Compression of strings with approximate repeats.
by L Allison - 1998 - Cited by 20 - Related articles
The model is further extended to include approximate reverse complementary repeats when analyzing DNA strings. Tests include the recovery of parameter ...
www.ncbi.nlm.nih.gov/pubmed/9783204
#
Compression of Strings with Approximate Repeats
by L Allison - Cited by 20 - Related articles
rection (reverse complementary repeats). These events can create repeats with lengths of hundreds ..... peats and two types of reverse complementary repeats ...
www.aaai.org/Papers/ISMB/1998/ISMB98-002.pdf

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Note added at 20 hrs (2010-10-10 16:05:28 GMT)
--------------------------------------------------

Hi again
I have found "inverted"
see

CIPO - Patent - 2286480
by D GRIERSON - 1998
inverted repeat sequence may be a synthetic polynucleotide sequence or ... le vecteur d'inhibition du gène une séquence répétée inverse de toute ou partie ...
patents.ic.gc.ca/details?patent_number=2286480 - Cached

this would fit in with "inversé", with an accute accent:)

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Note added at 20 hrs (2010-10-10 16:10:23 GMT)
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Fortunately I don't translate this type of stuff, but if I did I would be the first one to ask the client here.
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