English to Polish Medical Biology (-tech,-chem,micro-)

Compound inhibitory activity was measured is a radioactive enzyme assay. The buffer composition was adopted from Lisnock et al (Biochemistry, 1998, vol. 37, pp 16573-16581). Peptide substrate was selected from Chen et al (Biochemistry, 2000, vol. 39, 2079-2087). The concentrations of p38á, [ă33P-ATP] and peptide were equal 1 nM, 85 mm and 250 mM, respectively. Incorporation of 33P into peptide was measured using absorption on filtermats with subsequent wash with 100 mM phosphoric acid followed by ethanol.