This site uses cookies. Some of these cookies are essential to the operation of the site, while others help to improve your experience by providing insights into how the site is being used. For more information, please see the ProZ.com privacy policy.
Cookie settings
so quick bringing ... and laying
preposition

Glossary entry

English term or phrase:

5X SSC

English answer:

5x concentared (0.75M NaCl, 0.075M) sodium chloride and sodium citrate solution

Added to glossary by Veronica Prpic Uhing
Oct 31, 2005 17:23
18 yrs ago
5 viewers *
English term

5X SSC

English Medical Genetics
The probe was radiolabelled with a-32P-dCTP (3000 Ci/mmol, Amersham) by a random priming method. Hybridization was performed in 50% formamide, 5X SSC (0.75M NaCl, 0.075M sodium citrate), Denhardts solution (0.1% Ficoll, 0.1% polyvinylpyrrolidone, 0.l% BSA), and 0.l% SDS for 16 h at 42¡ãC.
Responses
5 +4 SSC" is sodium chloride and sodium citrate solution
5 +1 five times of suggested working concentration of SSC

Discussion

XiaoRan Nov 1, 2005:
I post the above words don't want to have the points back, but just indicate "Generally, professional's suggestion is right".
I myself had once choose a not-best answer, but after that I always think more than twice of a professional's suggestion....
XiaoRan Nov 1, 2005:
Please see http://www.fasebj.org/cgi/content/full/12/15/1693
"...It was then washed four times at room temperature for 15 min in 2x solution of sodium citrate (SSC) and 0.1% sodium dodecyl sulfate (SDS), heated to 48��C ..for 30 min, and then washed twic
XiaoRan Nov 1, 2005:
In addition, SSC is the abbreviation of "Solution of Sodium Citrate" NOT as VPUHING indicated.
XiaoRan Oct 31, 2005:
to VPUHING:
not theoretically yes, but practically yes. I have done research work in this field for 7 years, and I use 0.5X solution as a working buffer for millions of times. In addtion, sometimes we also use 2X solution as a working buffer.

Responses

+4
4 mins
Selected

SSC" is sodium chloride and sodium citrate solution

SSC is a abbreviation of sodium chloride and sodium citrate solution

5X SSC (0.75M NaCl, 0.075M sodium citrate)

--------------------------------------------------
Note added at 6 mins (2005-10-31 17:29:25 GMT)
--------------------------------------------------

5x means it is 5 x concentrated and hence, needs to be diluted 5x in working buffer

SSC" is a abbreviation of sodium chloride and sodium citrate solution, which used to transfer nuclear acid from gel to membrane ( 20 *SSC or 10*SSC or 5*SSC with alkaline). In Roche's DIG system, they said that the high solt concentration SSC shows much efficiently than the others like valcuum or electrophoresis.
http://www.protocol-online.org/archive/posts/3129.html



--------------------------------------------------
Note added at 58 mins (2005-10-31 18:21:36 GMT)
--------------------------------------------------

Note added at 6 mins (2005-10-31 17:29:25 GMT)
--------------------------------------------------

5x means it is 5 x concentrated and hence, needs to be diluted 5x into the working buffer


--------------------------------------------------
Note added at 5 hrs 26 mins (2005-10-31 22:49:55 GMT)
--------------------------------------------------

To XiaoRan: The real issue of importance here is the final concentrations of constituents in the assay. How you get there needs to be clarified ¨C 0.5 x of what, 2x of what, concentration of 5x (see above), especially when you decide to publish your data and communicate them to the others to understand and therefore better appreciate your work.

--------------------------------------------------
Note added at 7 hrs 10 mins (2005-11-01 00:33:34 GMT)
--------------------------------------------------

To XiaoRan: In this instance SSC is specified as: 5X SSC (0.75M NaCl, 0.075M sodium citrate),
Peer comment(s):
agree Ian M-H (X) : saline sodium citrate, salt sodium citrate or standard saline citrate
3 mins
Thank you Ian!
agree Freimanis
31 mins
Thank you!
neutral XiaoRan : yes. the explanation of SSC is very good. But solutions such as 0.5 X buffer can also be used as working buffer. 5X solution can be diluted 5 times into a working buffer (1X), but it can also be diluted 10 times to make a working buffer (0.5X)
1 hr
Theoretically yes, but in conventional and our lab 1X is the final concentration that you obtain from 5X in this instance, otherwise you would call initial concentrate 10X, 12X etc. Naturally, read your recipe carefully when using for the first time
agree Drunya : Hmm... I thought SSC stands for Saline sodium citrate, basically making it sodium citrate. Never knew it has sodium chloride too
2 hrs
0.75M NaCl is not saline, that is why. Once 5x SSC is diluted to 1x then you get saline sodium citrate. Namely, saline (physiologic solution) is 0.15M NaCl, which is 300 milliosmolar that is isotonic
agree Jörgen Slet
4 hrs
Thank you!
Something went wrong...
4 KudoZ points awarded for this answer. Comment: "Thanks!"
+1
10 mins

five times of suggested working concentration of SSC

.

--------------------------------------------------
Note added at 55 mins (2005-10-31 18:18:45 GMT)
--------------------------------------------------

Yes, SSC is an abbreviation, no need to translate it at all.
Yes, what in parenthesis explain the word just before it.
I think it's easy to understand this, so I did not said it. Sorry.
(What I explained is the key word that may confuse people.)

--------------------------------------------------
Note added at 6 hrs 58 mins (2005-11-01 00:21:47 GMT) Post-grading
--------------------------------------------------

SSC is the abbreviation of "Solution of Sodium Citrate".
Peer comment(s):
agree Jörgen Slet
4 hrs
Something went wrong...
Close search
Term search
  • All of ProZ.com
  • Term search
  • Jobs
  • Forums
  • Multiple search