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NV - Lab testing
Nov 6, 2003 22:32
20 yrs ago
1 viewer *
German term

c-GMP-SPA-TEST

German to English Medical Biology (-tech,-chem,micro-)
Platte3
c-GMP-SPA-TEST
21.01.1998
IEnzvm: PDEV I
IPlatteNr.: 3 I
Rohdaten
Proposed translations (English)
4 +1 cyclic guanosine monophosphate | scintillation proximity assay
3 +1 Current Good Manufacturing Practice - Staphylococcal Protein A Test
4 Leave it as it is or consult client.

Discussion

Drunya Nov 14, 2003:
We could probably be more helpful if you provide us with some additional context. What did they do the assay for?
Drunya Nov 14, 2003:
PDEV I is most probably a misspelled PDE VI, or phosphodiesterase IV that degrades cGMP.
Drunya Nov 14, 2003:
There is no doubt about cGMP; it is cyclic guanosine monophosphate, as suggested by Martinique.

Proposed translations

+1
14 hrs
Selected

cyclic guanosine monophosphate | scintillation proximity assay

It's the name of radioimmunoassay kit: cGMP-SPA BIOTRAK, Amersham Biosciences. It would be better to stay it as is.

http://jpet.aspetjournals.org/cgi/content/full/283/2/894

cGMP levels were then determined in samples, using a commercially available ***cGMP radioimmunoassay kit (BIOTRAK cGMP-SPA).


http://jpet.aspetjournals.org/cgi/content/full/302/1/127

[3H]cAMP, [3H]cGMP, [3H]rolipram, ***scintillation proximity assay (SPA)*** beads, and Ficoll-Paque were from Amersham Biosciences UK, Ltd. (Little Chalfont, Buckinghamshire, UK).

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Note added at 2003-11-16 15:37:07 (GMT)
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Here is the description of your analysis:

http://www.chromatography.amershambiosciences.com/aptrix/upp...

Phosphodiesterase [ 3H ]cGMP SPA enzyme assay
• Scintillation proximity assay (SPA)
• Measures either cAMP or cGMP dependent PDE isoenzymes
• Homogeneous assay, performed in microtitre plates or tubes
• Suitable for high throughput screening
• Ready to use buffer
• [3H] tracer included
The assay concept from Amersham Biosciences is based on the observation that linear nucleotides bind preferentially to SPA yttrium silicate beads compared to cyclic nucleotides in the presence of zinc sulphate. Therefore, under optimized conditions, the product of the enzyme reaction binds directly to the SPA beads, and the enzyme substrate will not.

This page also contains a pdf file with the detailed description.

HTH
Reference:

http://www.bioprocess.amershambiosciences.com/aptrix/upp00919.nsf/Content/Biotrak+Products+Second+Messengers

Peer comment(s):
agree Elena Ivaniushina : more likely
1 day 9 hrs
Thank you
Something went wrong...
4 KudoZ points awarded for this answer.
+1
29 mins

Current Good Manufacturing Practice - Staphylococcal Protein A Test


A possibility; cGMP is correct, the latter part (SPA-test) is an educated guess based on

http://www.cdc.gov/mmwr/preview/mmwrhtml/00001218.htm



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Note added at 2003-11-06 23:07:13 (GMT)
--------------------------------------------------

or, perhaps cGMP is an abbreviation for cyclic guanosine monophosphate, a metabolic regulator.

Pardon.

http://bric.postech.ac.kr/webzine/content/review/cellbio/feb...
Peer comment(s):
agree Zareh Darakjian Ph.D. : with cyclic guanosine monophosphate, of course...
4 hrs
Something went wrong...
3 days 8 hrs

Leave it as it is or consult client.

This is probably an international term (these things usually are), in which case it doesn't require translation. Unless, of course you are required to write it out in full which defeats the purpose of the abbreviated form.
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