Jun 14, 2018 17:24
5 yrs ago
3 viewers *
Spanish term
Mexico
Extinción
Spanish to English
Science
Chemistry; Chem Sci/Eng
Assays carried out on foods
Justo antes de inocular el ensayo, transferir una cantidad suficiente del cultivo preparado bajo (B.15.6.1) a un tubo de centrífuga estéril, centrifugar a 2600 rpm durante 5 min, decantar y resuspender el paquete celular en 10 ml de solución salina. Hacer dos lavados más. Transferir a una cubeta de 1 cm y efectuar la lectura en el espectrofotómetro a 575 nm. Estandarizar el cultivo a fin de obtener siempre aproximadamente la misma extinción. No debe olvidarse sustraer de la extinción del cultivo la del medio de ensayo, que es un medio coloreado.
Así pues, para una extinción del cultivo (B.15.6.1) entre 0,2 y 0,4 (después de sustraer la extinción propia del medio), introducir de 4 a 8 gotas de cultivo en el tubo que contiene 10 ml de medio (B.15.6.4). Si la extinción no se encuentra en la zona arriba mencionada, adaptar la dilución como sigue:
Así pues, para una extinción del cultivo (B.15.6.1) entre 0,2 y 0,4 (después de sustraer la extinción propia del medio), introducir de 4 a 8 gotas de cultivo en el tubo que contiene 10 ml de medio (B.15.6.4). Si la extinción no se encuentra en la zona arriba mencionada, adaptar la dilución como sigue:
Proposed translations
(English)
| 4 +2 | extincton or dilution |
liz askew
|
| 3 | depletion |
Andrew Felts
|
Proposed translations
+2
1 hr
Selected
extincton or dilution
Believe it or not, and I have really been searching (seriously)
http://www.asmscience.org/content/book/10.1128/9781555817770...
Finally, the chapter describes the extinction culture, which is also called dilution culture and dilution to extinction. It involves diluting water samples with filter-sterilized water until only a few bacteria remain and then growing the cells in either the unamended water or by adding small amounts of organic substrates to culture them.
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Note added at 1 hr (2018-06-14 18:51:20 GMT)
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sorry
extinction or dilution
aem.asm.org/content/59/3/881.abstract
by DK Button - 1993 - Cited by 424 - Related articles
Dilution culture, a method for growing the typical small bacteria from natural aquatic assemblages, ... Fatemeh Mohammadipanah et al., Pharmaceutical Biology ..... Novel Bacterium Isolated by Extinction Culture and Having Kinetic Constants ...
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Note added at 1 hr (2018-06-14 18:52:19 GMT)
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When Is a Microbial Culture “Pure”? Persistent Cryptic Contaminant ...
https://pdfs.semanticscholar.org/62a7/8a0127f5e4178a68cf110c...
by PM Shrestha - 2013 - Cited by 14 - Related articles
However, a culture in which KN400 could no longer be detected was obtained by serial dilution to extinction in liquid medium. The KN400-free culture could not ...
Environmental Microbiology
https://books.google.co.uk/books?isbn=0121828026
Jared R. Leadbetter - 2005 - Science
Isolating a pure culture often requires repeated dilution-to-extinction series, and it is recommended that a subsequent dilution series is initiated immediately with ...
High molecular weight dissolved organic matter enrichment selects for ...
https://www.nature.com › the isme journal › original article
by OA Sosa - 2015 - Cited by 15 - Related articles
15 May 2015 - To obtain microbial isolates capable of degrading semi-labile DOM for growth, we conducted dilution to extinction cultivation experiments using ...
http://www.asmscience.org/content/book/10.1128/9781555817770...
Finally, the chapter describes the extinction culture, which is also called dilution culture and dilution to extinction. It involves diluting water samples with filter-sterilized water until only a few bacteria remain and then growing the cells in either the unamended water or by adding small amounts of organic substrates to culture them.
--------------------------------------------------
Note added at 1 hr (2018-06-14 18:51:20 GMT)
--------------------------------------------------
sorry
extinction or dilution
aem.asm.org/content/59/3/881.abstract
by DK Button - 1993 - Cited by 424 - Related articles
Dilution culture, a method for growing the typical small bacteria from natural aquatic assemblages, ... Fatemeh Mohammadipanah et al., Pharmaceutical Biology ..... Novel Bacterium Isolated by Extinction Culture and Having Kinetic Constants ...
--------------------------------------------------
Note added at 1 hr (2018-06-14 18:52:19 GMT)
--------------------------------------------------
When Is a Microbial Culture “Pure”? Persistent Cryptic Contaminant ...
https://pdfs.semanticscholar.org/62a7/8a0127f5e4178a68cf110c...
by PM Shrestha - 2013 - Cited by 14 - Related articles
However, a culture in which KN400 could no longer be detected was obtained by serial dilution to extinction in liquid medium. The KN400-free culture could not ...
Environmental Microbiology
https://books.google.co.uk/books?isbn=0121828026
Jared R. Leadbetter - 2005 - Science
Isolating a pure culture often requires repeated dilution-to-extinction series, and it is recommended that a subsequent dilution series is initiated immediately with ...
High molecular weight dissolved organic matter enrichment selects for ...
https://www.nature.com › the isme journal › original article
by OA Sosa - 2015 - Cited by 15 - Related articles
15 May 2015 - To obtain microbial isolates capable of degrading semi-labile DOM for growth, we conducted dilution to extinction cultivation experiments using ...
4 KudoZ points awarded for this answer.
Comment: "Thank you"
26 mins
Andrew Felts
United States
United States
Native in English
United States
proficient and precise translations
Native in: English (Variant: US)
Works in: Spanish to English, Portuguese to English
depletion
I think depletion would be the best gloss in this context because extinction doesn't make sense and after searching for some articles online about centrifuges I found that depletion is more commonly used terminology to describe chemicals used up in a centrifuge.
Example sentence:
It is fed into centrifuges that enrich the depleted material back up to the original concentration and then fed back into the enriching cascade.
Discussion
According to Thrash, the technique involves “dilution to extinction”—diluting a microbial sample into sterilized seawater such that each well of a 96-well plate contains, on average, a single cell. Then, after a few weeks of culture in nutrient-poor media, the team measures growth by looking for a boost in cell numbers.
https://www.fda.gov/ucm/groups/fdagov-public/@fdagov-foods.....
inoculated samples should occur, the LOD Working Group has proposed an alternative challenge procedure, Dilution to Extinction (DTE). This methodology ... of the matrix by a cultural reference method. In certain ... replicate in culture media.