many hits on Google ... DNA was isolated from blood and bronchial lavage using the QIAamp 96 DNA Blood
and DNeasy 96 Tissue Kits, respectively. ... B: blood; BL: bronchial lavage

Red cells in additive solution, buffy coat removed (AS-BCR) were prepared from CPD whole blood (63 + 450 ±10 % ml) by a modified "Amsterdam technique", on the day of collection. RBC stored at + 4 ± 2°C for up to 10 days, were washed twice in isotonic saline solution(300-300±20 ml,+20°C). The diluted cells were then centrifuged in a Jouan 5,22 (France) centrifuge at 3500rpm, +20 °C. The supernatant was removed and washed RBCs were resuspended in additive solution. The per cent recovery of hemoglobin after washing was 88,3. The average prewash IgA was 35,2 mg/unit with the postwash average of 0.17 mg/unit. During the process an average 38,2 ml of AS-BCR was lost. After process natural antibodies were not detected by blood group serological methods. Our results have led to the conclusion that two sequantial washings seem to guarantee an IgA level of less than 0.2 mg/ unit and a total protein content of less than 0.5 g/unit to meet the recommendations of the Guide of Council of Europe