English to Italian Other Medical (general)

After 24 h +/2 spermidine primary human keratinocytes were
trypsinised, washed with PBS and fixed in 70% ethanol at 4uC
overnight. Cells were resuspended in 50 mg/ml propidium
iodide(PI) (Sigma-Aldrich), 100 mg RNaseA (Sigma-Aldrich),
3.8 mM sodium citrate in PBS and incubated at 4uC over night.
For each sample 100000 cells were analysed using a LSRII flow
cytometer with FACSDiva software (v 5.1.03) (both BD Biosciences,
CA USA). The detector was set to linear, PI excitation at
488 nm and emission collected in the 610–620 channel, cells were
gated on width and area to exclude doublets.